小鼠抗PCBP1單克隆抗體

Background:

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008].

Applications:

WB, IHC

Name of antibody:

PCBP1

Immunogen:

Fusion protein of human PCBP1

Full name:

poly(rC) binding protein 1 (PCBP1)

Synonyms:

HEL-S-85; hnRNP-E1; hnRNP-X; HNRPE1; HNRPX

SwissProt:

Q15365

IHC positive control:

carcinoma of human kidney tissue and adenocarcinoma of human ovary tissue; carcinoma of human pancreas tissue and human prostate tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

37 kDa

WB Positive control:

Hela, HepG2, K562, MCF-7, SVT2 cell, Human liver tissue, Human ovary tissue, Human brain tissue lysates

WB Recommended dilution:

500-2000