兔抗AKT1(Ab-129) 多克隆抗體

Background:

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase.

Applications:

WB, IHC, IF

Name of antibody:

AKT1(Ab-129)

Immunogen:

Synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of serine 129 (D-N-S(p)-G-A).

Full name:

v-akt murine thymoma viral oncogene homolog 1

Synonyms：

AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

SwissProt:

P31749

IHC positive control:

Human skeletal muscle tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

56 kDa

WB Positive control:

Huvec cells treated with Serum (30%, 30mins) lysate

WB Recommended dilution:

500-3000

IF positive control:

Hela cells

IF Recommend dilution:

100-500