小鼠抗KPNB1單克隆抗體

Background:

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene.

Applications:

WB, IHC

Name of antibody:

KPNB1

Immunogen:

Fusion protein of human KPNB1

Full name:

karyopherin subunit beta 1

Synonyms:

IMB1; IPO1; IPOB; Impnb; NTF97

SwissProt:

Q14974

IHC positive control:

Human adult heart tissue

IHC Recommend dilution:

500-2000

WB Predicted band size:

97 KD

WB Positive control:

Hela, A431, NIH/3T3, PC12 cell lysates

WB Recommended dilution:

500-2000