小鼠抗MRPL44單克隆抗體

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.

Applications:

WB, IHC

Name of antibody:

MRPL44

Immunogen:

Fusion protein of human MRPL44

Full name:

mitochondrial ribosomal protein L44

Synonyms:

L44MT; COXPD16; MRP-L44

SwissProt:

Q9H9J2

IHC positive control:

Human colorectal cancer and Human thyroid cancer; Human endometrial cancer and Human prostate cancer

IHC Recommend dilution:

200-500

WB Predicted band size:

38 KD

WB Positive control:

Jurkat, K562, A549, MCF7, Hela, HepG2, 293T cell lysates

WB Recommended dilution:

200-1000